Enzyme activity Essay

Purpose The purpose of this lab is to canvass for enzyme act, look at enzyme specificity, and how temperature affects enzyme activity.Time essential to perform this lab near 3 hours conceptualisationFIRST Read the lab in its totality TWICE before you begin. You will perform the experiment, write your lab reputation and include the answers to the supplyitional 4 questions within the text for in unspoilt consultation on this experiment.Materials3% hydrogen hydrogen peroxidea ho implementhold blender stump spudes (3 medium size stump spudes, that are peeled and quartered) topple weewee8 stress renders8 switch pipetssugar packetssalta dainty sauce scrapa refrigerator with a freezerstove4 substantiate plastic formstongs (to remove taste tubes from boiling water)4 coffee cupscheesecloth to filter the potato extracta wax draw or Sharpie markera normalscale or sense of balance250 ml graduated cylinderoven mitt or tongs preparedness cadence 1 Research (online) the re action between catalase and hydrogen peroxide and be familiar with the reactants, products, and enzyme. You will include the overall reaction in your lab report for this experiment. We will measure enzyme activity by beat the superlative of the card-houses produced.Questions1.Given your research, why are we bill the height of the bubbles produced to indicate how frequently enzyme activity has occurred? 2.What product are we specifically observing being produced?Preparation Step 21.Prepare the potato extract (catalase) using the following technique peel 3 medium size potatoes and cut them into quarters work the potatoes to the blender with 250 mls of tap water to cover the potatoes and blend on superior until the potatoes are pureed (blended to a thick eloquent or paste) place a cheesecloth over a container and use the cheesecloth to obtain the eloquent portion (only) of your potato puree. You can squeeze the cheesecloth in the end to obtain the final portions of the liq uid from the puree. 1.Make a 1% sucrose resultant using the method place in your osmosis lab. 2.Make a 1% salt resolving power by measuring 1 gram of salt using your scale or balance, add this to your graduated cylinder and accordingly fill to the 100ml mark. You can then transfer this mixture to a small saucepan, heat and stir until the mixture is dissolved. single-valued functionExperiment 1 Testing for Enzymatic ActivityFor this experiment, you will need4 canvass tubesrulerwater1% sucrose (sugar) dissolver1% salt base3% hydrogen peroxidecatalase (the liquid portion of your potato extract)4 clear plastic cupswax penciltransfer pipetsStep 1 Add 3mls of the following solutions to a denominate test tube using a fresh transfer pipet with each new solutiontest tube 1 tap watertest tube 2 1% sucrose solutiontest tube 3 1% salt solutiontest tube 4 3% hydrogen peroxideQuestions1.Why is it important to use a fresh transfer pipet for each new solution? Step 2 Add 2 mls of the catala se solution (liquid portion of the potato extract) to each test tube and place into a plastic cup to keep it upright. Pick up each test tube, tap the get through/ swirl the contents and then using your ruler record in plug-in 1 the bubble height (if any) that is produced. Remember to include this duck (with a strong title) in your lab report.Experiment 2- Testing the proceeds of Temperature on Enzyme ActivityFor this experiment, you will need4 test tubesruler3% hydrogen peroxidecatalase (the liquid portion of your potato extract)4 coffee cupswax penciltransfer pipetsrefrigerator w/ freezerstovesaucepantap wateroven mitt or tongsStep 1 Label the test tubes 1, 2, 3 and 4, where test tube 1 represents the sample placed on the counter at room temperature, test tube 2 represents the tube placed in the refrigerator, test tube 3 represents the tube placed in the freezer, and test tube 4 represents the sample exposed to boiling water. You will expose catalase to each of these quadruple conditions.Questions4.Before you begin, predict under which treatment you expect to see the most enzyme activity and explain why you think that will happen. Step 2 post a saucepan with tap water on a burner and bring to a boil.Step 3 Use the thermometer to identify and record the temperature for room temperature, in your refrigerator, in your freezer, and then research the temperature of boiling water (do not coin this temperature) and record them in Table 2.Step 4 Place the test tubes into separate coffee cups to maintain the upward position. Add 2 mls of the catalase solution to each of the test tubes and then place tubes1, 2, and 3 in the conditions expound above. For test tube 4, fill the coffee mug half full of boiling water and then place test tube 4 in this container. Do not place the test tube into the pan of boiling water.Step 5 Allow the tubes to sit for 5 minutes, then remove the tubes from the conditions mentioned above (note be careful to use an oven mitt or tongs t o remove the test tube exposed to boiling water from the coffee cup).Step 6 Then add 3 mls of 3% hydrogen peroxide, swirl all tubes, and measure the bubbles produced by measuring bubble height (if any are produced). Record this data in Table 2.ResultsPlease include your results in two tables that are clearly tagged and have columns for the contents of each test tube, the treatment if applicable (experiment 2) and the height of the bubbles produced (if any).ConclusionsFor this portion, include the results obtained and an interpretation of the results. Also state whether the results were expected or not and, if the results are unexpected, include possible reasons for this and what your next step should be. Remember to as well as include your answers to the 4 Questions embedded in the experiment above for full credit.Submission Create your lab report using word-processing software, such as Microsoft Word and save it to your computer as a .doc, .docx, or .rtf file. To inject the repo rt, choose the link titled, Lab 6 Enzymes, above. Use the Browse My computer button in the Attach File area to attach your document. Be sure to complete your submission by choosing the Submit button at the bottom of the screen.